enascas12a prda 174 (Addgene inc)
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enascas12a prda 174
Enascas12a Prda 174, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enascas12a/pRDA_174+(Plasmid+%23136476)/bio_rxiv__64898__2026__02__15__704070-195-6-11
Average 93 stars, based on 26 article reviews
Enascas12a Prda 174, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enascas12a/pRDA_174+(Plasmid+%23136476)/bio_rxiv__64898__2026__02__15__704070-195-6-11
Average 93 stars, based on 26 article reviews
enascas12a prda 174 - by Bioz Stars,
2026-09
93/100 stars
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Activity Assay:Article Title: Optimized metrics for orthogonal combinatorial CRISPR screens. Article Snippet: :(0123456789) Scientific Reports | (2023) 13:7405 | https://doi.org/10.1038/s41598-023-34597-8 neo (Addgene: 98292). .. Then, a single clone with high SpCas9 activity was selected and transduced with lentiviral particles generated with the pRDA_174 (Addgene: 136476) Transduction:Article Title: Optimized metrics for orthogonal combinatorial CRISPR screens. Article Snippet: :(0123456789) Scientific Reports | (2023) 13:7405 | https://doi.org/10.1038/s41598-023-34597-8 neo (Addgene: 98292). .. Then, a single clone with high SpCas9 activity was selected and transduced with lentiviral particles generated with the pRDA_174 (Addgene: 136476) Generated:Article Title: Optimized metrics for orthogonal combinatorial CRISPR screens. Article Snippet: :(0123456789) Scientific Reports | (2023) 13:7405 | https://doi.org/10.1038/s41598-023-34597-8 neo (Addgene: 98292). .. Then, a single clone with high SpCas9 activity was selected and transduced with lentiviral particles generated with the pRDA_174 (Addgene: 136476) Expressing:Article Title: Optimized metrics for orthogonal combinatorial CRISPR screens. Article Snippet: :(0123456789) Scientific Reports | (2023) 13:7405 | https://doi.org/10.1038/s41598-023-34597-8 neo (Addgene: 98292). .. Then, a single clone with high SpCas9 activity was selected and transduced with lentiviral particles generated with the pRDA_174 (Addgene: 136476) Article Title: Benchmarking Perturbation Tools for the Noncoding Genome Article Snippet: .. For Article Title: A CRISPR-based SARS-CoV-2 diagnostic assay that is robust against viral evolution and RNA editing Article Snippet: .. The pET28b-T7-Cas12a-NLS-6xHis expression plasmids were gifts from Keith Joung and Benjamin Kleinstiver (Addgene plasmid #114069 [AsCas12a], #114070 [LbCas12a], #114072 [ Article Title: An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing Article Snippet: .. The pET28b-T7-Cas12a-NLS-6xHis expression plasmids were gifts from Keith Joung and Benjamin Kleinstiver (Addgene plasmid #114069 [AsCas12a], #114070 [LbCas12a], #114072 [ Construct:Article Title: Optimized metrics for orthogonal combinatorial CRISPR screens. Article Snippet: :(0123456789) Scientific Reports | (2023) 13:7405 | https://doi.org/10.1038/s41598-023-34597-8 neo (Addgene: 98292). .. Then, a single clone with high SpCas9 activity was selected and transduced with lentiviral particles generated with the pRDA_174 (Addgene: 136476) Transfection:Article Title: COPII collar defines the boundary between ER and ER exit site and does not coat cargo containers Article Snippet: PCR was performed on plasmid pMaCTag-P13 (Addgene; #120024), kindly provided by Marius Lemberg (Zentrum für Molekulare Biologie der Universität Heidelberg, Heidelberg, Germany), using oligos M1_SEC13, 5′-GTT GAT GGG CAG TGG GTG TGC ATC AGT GAT GTC AAC AAG GGC CAG GGC TCC GTA TCA GCA TCA GTG ACA GAG GGC CAG CAG AAC GAG CAG TCA GGT GGA GGA GGT AGT G-3′ and M2_Sec13_, 5′-CAG GAA GGG GCA GTC CTG GAG CTG GCG GGT GGG GAG CCA GGC CCC ACC TGT CTT GAA AAA AAG CAG AAC GAG CAG TGA CAA ATC TAC AAG AGT AGA AAT TAG CTA GCT GCA TCG GTA CC-3′. .. Cells were transfected with purified PCR product, and Purification:Article Title: COPII collar defines the boundary between ER and ER exit site and does not coat cargo containers Article Snippet: PCR was performed on plasmid pMaCTag-P13 (Addgene; #120024), kindly provided by Marius Lemberg (Zentrum für Molekulare Biologie der Universität Heidelberg, Heidelberg, Germany), using oligos M1_SEC13, 5′-GTT GAT GGG CAG TGG GTG TGC ATC AGT GAT GTC AAC AAG GGC CAG GGC TCC GTA TCA GCA TCA GTG ACA GAG GGC CAG CAG AAC GAG CAG TCA GGT GGA GGA GGT AGT G-3′ and M2_Sec13_, 5′-CAG GAA GGG GCA GTC CTG GAG CTG GCG GGT GGG GAG CCA GGC CCC ACC TGT CTT GAA AAA AAG CAG AAC GAG CAG TGA CAA ATC TAC AAG AGT AGA AAT TAG CTA GCT GCA TCG GTA CC-3′. .. Cells were transfected with purified PCR product, and Polymerase Chain Reaction:Article Title: COPII collar defines the boundary between ER and ER exit site and does not coat cargo containers Article Snippet: PCR was performed on plasmid pMaCTag-P13 (Addgene; #120024), kindly provided by Marius Lemberg (Zentrum für Molekulare Biologie der Universität Heidelberg, Heidelberg, Germany), using oligos M1_SEC13, 5′-GTT GAT GGG CAG TGG GTG TGC ATC AGT GAT GTC AAC AAG GGC CAG GGC TCC GTA TCA GCA TCA GTG ACA GAG GGC CAG CAG AAC GAG CAG TCA GGT GGA GGA GGT AGT G-3′ and M2_Sec13_, 5′-CAG GAA GGG GCA GTC CTG GAG CTG GCG GGT GGG GAG CCA GGC CCC ACC TGT CTT GAA AAA AAG CAG AAC GAG CAG TGA CAA ATC TAC AAG AGT AGA AAT TAG CTA GCT GCA TCG GTA CC-3′. .. Cells were transfected with purified PCR product, and Plasmid Preparation:Article Title: Benchmarking Perturbation Tools for the Noncoding Genome Article Snippet: .. For Article Title: A CRISPR-based SARS-CoV-2 diagnostic assay that is robust against viral evolution and RNA editing Article Snippet: .. The pET28b-T7-Cas12a-NLS-6xHis expression plasmids were gifts from Keith Joung and Benjamin Kleinstiver (Addgene plasmid #114069 [AsCas12a], #114070 [LbCas12a], #114072 [ Article Title: An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing Article Snippet: .. The pET28b-T7-Cas12a-NLS-6xHis expression plasmids were gifts from Keith Joung and Benjamin Kleinstiver (Addgene plasmid #114069 [AsCas12a], #114070 [LbCas12a], #114072 [ |